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Provide updated information on how to download course data
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susannasiebert committed Jul 5, 2023
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24 changes: 22 additions & 2 deletions 02-prerequisites.Rmd
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Expand Up @@ -68,11 +68,31 @@ install.packages("shinycssloaders", dependencies=TRUE)

For this course, we have put together a set of input data for the HCC1395
cell line. Data from this cell line is commonly used as test data in bioinformatics
applications.
applications. The input data consists of the following files:

For pVACseq:
- `annotated.expression.vcf.gz`: A somatic (tumor-normal) VCF and its tbi index file. The VCF has been
annotated with VEP and has coverage and expression information added and.
- `phased.vcf.gz`: A phased tumor-germline VCF and its tbi index file to provide information about
in-phase proximal variants that might alter the predicted peptide sequence around a somatic
mutation of interest
- `optitype_normal_result.tsv`: A OptiType file with HLA allele typing predictions

For pVACfuse:
- `agfusion_results`: A AGFusion output directory with annotated fusion calls
- `star-fusion.fusion_predictions.tsv`: A STARFusion prediction file with fusion read support
and expression information

General:
- `Homo_sapiens.GRCh38.pep.all.fa.gz`: A reference proteome peptide FASTA to use
for determining whether there are any reference matches of neoantigen candidates

To download this data, please run the following command:

```{r, engine = 'bash', eval = FALSE}
TODO: download instructions
wget
https://raw.githubusercontent.com/griffithlab/pVACtools_Intro_Course/main/HCC1395_inputs.zip
unzip HCC1395_inputs.zip
```

This course will not cover the required pre-processing steps for the pVACtools
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