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Single cell gene expression atlas of human pancreatic islets

This repository contains R scripts for R shiny web application to browse single cell gene expression data from Shrestha et al., JCI Insight(2021) doi:10.1172/jci.insight.151621. This expansive dataset contains ~45,000 cells from 5 donor pancreatic islets and provides a comprehensive map of human islet biology. It will be an extremely useful resource that complements existing single cell datasets from human islets.

Installation

The web app can be accessed at https://powersbrissovalab.shinyapps.io/scRNAseq-Islets/ or the web app can run locally through app.R

Note about data

The data (stored as .rda file) was too large for Github's single file limit, so it was chunked into 2 small files using the 7zip utility. To re-generate the data file, please use 7zip to extract the data DATA/Islets2.Rda.zip.001 through DATA/Islets2.Rda.zip.003. 7zip will extract to Islets2.Rda, which is what the R Shiny app expects. You may then delete the chunked files.


Page last edited on 2021-07-22