dSTORM microscopy can improve the resolution of conventional fluorescent microscopy by one order of magnitude. The data analysis software has to localize many thousand single molecule point-spread-functions to reconstruct an image of the underlying structure.
Content of this archive: storm/ sources for single-molecule-localization and command-line utility to analyze .sif and .hdf5 input data gui/ Qt-based graphical-user-interface (GUI) for selection of input files and appropriate parameter(s) python/ helper scripts to analyze and display the coordinate lists generated by the utilities above colorcomposer/ multi-channel measurements have to be aligned due to chromatic aberrations. Tetraspeck-Beads are detected automatically so that the images can be registrated easily.
A manual is in doc/doc.pdf.
See INSTALL for instructions on how to build and install storm or storm-gui from scratch.
Download binaries for Windows 64-bit from http://hci.iwr.uni-heidelberg.de/Software/simpleSTORM-win64.zip
More algorithmic details are available in the diploma thesis at http://hci.iwr.uni-heidelberg.de/Staff/jschleic/
Copyright 2010-2013 by Ullrich Koethe, Joachim Schleicher, Frank Herrmannsdoerfer and Ilia Kats
Please direct questions, bug reports, and contributions to ullrich.koethe (AT) iwr.uni-heidelberg.de