diff --git a/README.md b/README.md index edfec71..36ed4f6 100644 --- a/README.md +++ b/README.md @@ -12,7 +12,7 @@ This classifier is suitable for coarsely classifying 18S sequences to order or c Created from the SILVA 138 SSU Ref Nr99 (Preusse et al., 2007), the most recent curated dataset, with modifications described below. -We checked for the presence of species with more than one unique taxonomic lineage using the check_for_SILVA_inconsistencies4.plx script in the CheckSilva138Taxonomy directory. We found that 2,495 / 98,776 (2.5%) *unique* species were annotated with more than one taxonomic lineage due to the way SILVA curates taxonomic lineages using an automated phylogeny-based method (combined with manual curation for certain groups). This affects 335,983 / 510,984 (66%) of all the sequneces in the SILVA fasta file. +We checked for the presence of species with more than one unique taxonomic lineage using the check_for_SILVA_inconsistencies.plx script in the CheckSilva138Taxonomy directory. We found that 2,495 / 98,776 (2.5%) *unique* species were annotated with more than one taxonomic lineage due to the way SILVA curates taxonomic lineages using an automated phylogeny-based method (combined with manual curation for certain groups). This affects 335,983 / 510,984 (66%) of all the sequneces in the SILVA fasta file. This version of the 18S classifier has been trained to make assignments to the **genus** rank only. This was done for 3 reasons: